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Acute inhibition of Carboxypeptidase (CPE) decreases hyperexcitability in TeNT-injected mice. (A) Left: diagram of experimental design: a <t>16-channel</t> silicone probe was used to record LFP in different layers of the primary visual cortex, channels were analyzed in three groups according to their recording sites in relation to the surface of the cortex: the five most superficial, the five deepest, and the six intermediate channels. GEMSA was applied locally to inhibit CPE activity. Right: Examples of LFP traces obtained with a 16-channels probe from the visual cortex of an Acute epileptic mouse. (B) LFP traces of an Acute epileptic mouse before (baseline, top) and after GEMSA administration at two different time points: early (5–10 min) and late (10–20 min). (C) Coastline analysis of LFP signals recorded before (baseline) and after GEMSA administration at early and late time points. The analysis was differentially performed for superficial (left, red), intermediate (middle, green), and deep (right, blue) channels (Two-way ANOVA, Channel factor p > 0.05, Time factor p < 0.001; Baseline vs. Early: p < 0.01, Baseline vs. Late: p < 0.001, Early vs. Late: p < 0.001, n = 4). The mean, SEM, and value of individual recordings are shown for each group. *** p < 0.001.
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Acute inhibition of Carboxypeptidase (CPE) decreases hyperexcitability in TeNT-injected mice. (A) Left: diagram of experimental design: a <t>16-channel</t> silicone probe was used to record LFP in different layers of the primary visual cortex, channels were analyzed in three groups according to their recording sites in relation to the surface of the cortex: the five most superficial, the five deepest, and the six intermediate channels. GEMSA was applied locally to inhibit CPE activity. Right: Examples of LFP traces obtained with a 16-channels probe from the visual cortex of an Acute epileptic mouse. (B) LFP traces of an Acute epileptic mouse before (baseline, top) and after GEMSA administration at two different time points: early (5–10 min) and late (10–20 min). (C) Coastline analysis of LFP signals recorded before (baseline) and after GEMSA administration at early and late time points. The analysis was differentially performed for superficial (left, red), intermediate (middle, green), and deep (right, blue) channels (Two-way ANOVA, Channel factor p > 0.05, Time factor p < 0.001; Baseline vs. Early: p < 0.01, Baseline vs. Late: p < 0.001, Early vs. Late: p < 0.001, n = 4). The mean, SEM, and value of individual recordings are shown for each group. *** p < 0.001.
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Acute inhibition of Carboxypeptidase (CPE) decreases hyperexcitability in TeNT-injected mice. (A) Left: diagram of experimental design: a <t>16-channel</t> silicone probe was used to record LFP in different layers of the primary visual cortex, channels were analyzed in three groups according to their recording sites in relation to the surface of the cortex: the five most superficial, the five deepest, and the six intermediate channels. GEMSA was applied locally to inhibit CPE activity. Right: Examples of LFP traces obtained with a 16-channels probe from the visual cortex of an Acute epileptic mouse. (B) LFP traces of an Acute epileptic mouse before (baseline, top) and after GEMSA administration at two different time points: early (5–10 min) and late (10–20 min). (C) Coastline analysis of LFP signals recorded before (baseline) and after GEMSA administration at early and late time points. The analysis was differentially performed for superficial (left, red), intermediate (middle, green), and deep (right, blue) channels (Two-way ANOVA, Channel factor p > 0.05, Time factor p < 0.001; Baseline vs. Early: p < 0.01, Baseline vs. Late: p < 0.001, Early vs. Late: p < 0.001, n = 4). The mean, SEM, and value of individual recordings are shown for each group. *** p < 0.001.
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Acute inhibition of Carboxypeptidase (CPE) decreases hyperexcitability in TeNT-injected mice. (A) Left: diagram of experimental design: a <t>16-channel</t> silicone probe was used to record LFP in different layers of the primary visual cortex, channels were analyzed in three groups according to their recording sites in relation to the surface of the cortex: the five most superficial, the five deepest, and the six intermediate channels. GEMSA was applied locally to inhibit CPE activity. Right: Examples of LFP traces obtained with a 16-channels probe from the visual cortex of an Acute epileptic mouse. (B) LFP traces of an Acute epileptic mouse before (baseline, top) and after GEMSA administration at two different time points: early (5–10 min) and late (10–20 min). (C) Coastline analysis of LFP signals recorded before (baseline) and after GEMSA administration at early and late time points. The analysis was differentially performed for superficial (left, red), intermediate (middle, green), and deep (right, blue) channels (Two-way ANOVA, Channel factor p > 0.05, Time factor p < 0.001; Baseline vs. Early: p < 0.01, Baseline vs. Late: p < 0.001, Early vs. Late: p < 0.001, n = 4). The mean, SEM, and value of individual recordings are shown for each group. *** p < 0.001.
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Acute inhibition of Carboxypeptidase (CPE) decreases hyperexcitability in TeNT-injected mice. (A) Left: diagram of experimental design: a <t>16-channel</t> silicone probe was used to record LFP in different layers of the primary visual cortex, channels were analyzed in three groups according to their recording sites in relation to the surface of the cortex: the five most superficial, the five deepest, and the six intermediate channels. GEMSA was applied locally to inhibit CPE activity. Right: Examples of LFP traces obtained with a 16-channels probe from the visual cortex of an Acute epileptic mouse. (B) LFP traces of an Acute epileptic mouse before (baseline, top) and after GEMSA administration at two different time points: early (5–10 min) and late (10–20 min). (C) Coastline analysis of LFP signals recorded before (baseline) and after GEMSA administration at early and late time points. The analysis was differentially performed for superficial (left, red), intermediate (middle, green), and deep (right, blue) channels (Two-way ANOVA, Channel factor p > 0.05, Time factor p < 0.001; Baseline vs. Early: p < 0.01, Baseline vs. Late: p < 0.001, Early vs. Late: p < 0.001, n = 4). The mean, SEM, and value of individual recordings are shown for each group. *** p < 0.001.
Omniplex Neural Recording System, supplied by plexon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Acute inhibition of Carboxypeptidase (CPE) decreases hyperexcitability in TeNT-injected mice. (A) Left: diagram of experimental design: a <t>16-channel</t> silicone probe was used to record LFP in different layers of the primary visual cortex, channels were analyzed in three groups according to their recording sites in relation to the surface of the cortex: the five most superficial, the five deepest, and the six intermediate channels. GEMSA was applied locally to inhibit CPE activity. Right: Examples of LFP traces obtained with a 16-channels probe from the visual cortex of an Acute epileptic mouse. (B) LFP traces of an Acute epileptic mouse before (baseline, top) and after GEMSA administration at two different time points: early (5–10 min) and late (10–20 min). (C) Coastline analysis of LFP signals recorded before (baseline) and after GEMSA administration at early and late time points. The analysis was differentially performed for superficial (left, red), intermediate (middle, green), and deep (right, blue) channels (Two-way ANOVA, Channel factor p > 0.05, Time factor p < 0.001; Baseline vs. Early: p < 0.01, Baseline vs. Late: p < 0.001, Early vs. Late: p < 0.001, n = 4). The mean, SEM, and value of individual recordings are shown for each group. *** p < 0.001.
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Image Search Results


Acute inhibition of Carboxypeptidase (CPE) decreases hyperexcitability in TeNT-injected mice. (A) Left: diagram of experimental design: a 16-channel silicone probe was used to record LFP in different layers of the primary visual cortex, channels were analyzed in three groups according to their recording sites in relation to the surface of the cortex: the five most superficial, the five deepest, and the six intermediate channels. GEMSA was applied locally to inhibit CPE activity. Right: Examples of LFP traces obtained with a 16-channels probe from the visual cortex of an Acute epileptic mouse. (B) LFP traces of an Acute epileptic mouse before (baseline, top) and after GEMSA administration at two different time points: early (5–10 min) and late (10–20 min). (C) Coastline analysis of LFP signals recorded before (baseline) and after GEMSA administration at early and late time points. The analysis was differentially performed for superficial (left, red), intermediate (middle, green), and deep (right, blue) channels (Two-way ANOVA, Channel factor p > 0.05, Time factor p < 0.001; Baseline vs. Early: p < 0.01, Baseline vs. Late: p < 0.001, Early vs. Late: p < 0.001, n = 4). The mean, SEM, and value of individual recordings are shown for each group. *** p < 0.001.

Journal: Frontiers in Cellular Neuroscience

Article Title: Synaptic Vesicles Dynamics in Neocortical Epilepsy

doi: 10.3389/fncel.2020.606142

Figure Lengend Snippet: Acute inhibition of Carboxypeptidase (CPE) decreases hyperexcitability in TeNT-injected mice. (A) Left: diagram of experimental design: a 16-channel silicone probe was used to record LFP in different layers of the primary visual cortex, channels were analyzed in three groups according to their recording sites in relation to the surface of the cortex: the five most superficial, the five deepest, and the six intermediate channels. GEMSA was applied locally to inhibit CPE activity. Right: Examples of LFP traces obtained with a 16-channels probe from the visual cortex of an Acute epileptic mouse. (B) LFP traces of an Acute epileptic mouse before (baseline, top) and after GEMSA administration at two different time points: early (5–10 min) and late (10–20 min). (C) Coastline analysis of LFP signals recorded before (baseline) and after GEMSA administration at early and late time points. The analysis was differentially performed for superficial (left, red), intermediate (middle, green), and deep (right, blue) channels (Two-way ANOVA, Channel factor p > 0.05, Time factor p < 0.001; Baseline vs. Early: p < 0.01, Baseline vs. Late: p < 0.001, Early vs. Late: p < 0.001, n = 4). The mean, SEM, and value of individual recordings are shown for each group. *** p < 0.001.

Article Snippet: Before the beginning of the recording, the electrode was allowed to settle for about 10 min. Local Field Potentials (LFP) signals were acquired at 1 kHz and bandpass filtered (0.3–200 Hz) with a 16-channel Omniplex recording system (Plexon, Dallas, TX).

Techniques: Inhibition, Injection, Activity Assay